Adeno-Associated Viral Titer in minutes using Nanoparticle Tracking Analysis
- Nanoparticle Tracking Analysis Characterization of Gold-Labeled Adeno Associated Virus (AAV) and Other Small Viruses
- NTA is an analytical tool that measures the size and concentration of virus particles
- NTA can measure virus titer in about 5 minutes for viruses above Dh ~35 nm
- AAV (Dh ~ 25 nm) is too small and does not scatter enough light to be measured directly by NTA
- Gold-labeled AAV enhances the light scattering and enables NTA characterization to rapidly measure AAV particle concentration and aggregation state
